PrimePCR™ Probe Assay: NIPSNAP3A, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051978
Assay Design:   exonic
Chromosome Location:   9:107521640-107521788question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000374767

Gene Information

NIPSNAP3A belongs to a family of proteins with putative roles in vesicular transport (Buechler et al. 2004 [PubMed 15177564]).[supplied by OMIM Mar 2008]

Gene Symbol:   NIPSNAP3A
Gene Name:   nipsnap homolog 3A (C. elegans)
Aliases:   DKFZp564D177, FLJ13953, MGC14553, NIPSNAP4, TASSC
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000136783
Entrez:   25934
UniGene:   Hs.530275
Chromosome Mapping:   9q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.240000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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