PrimePCR™ Probe Assay: TTYH3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TTYH3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TTYH3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051949
Assay Design:   exonic
Chromosome Location:   7:2696098-2697939question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000449107 ENST00000303498 ENST00000437172 ENST00000383778 ENST00000568225 ENST00000229003 ENST00000422538 ENST00000545824 ENST00000542202 ENST00000261716 ENST00000536202 ENST00000258796 ENST00000407643 ENST00000403167 ENST00000429448 ENST00000379749 ENST00000379747 ENST00000379743 ENST00000379742 ENST00000541179 ENST00000541481 ENST00000335551 ENST00000520002 ENST00000602557 ENST00000537824 ENST00000335312 ENST00000373091 ENST00000338316 ENST00000537121 ENST00000525985 ENST00000328544 ENST

Gene Information

This gene encodes a member of the tweety family of proteins. Members of this family function as chloride anion channels. The encoded protein functions as a calcium(2+)-activated large conductance chloride(-) channel. [provided by RefSeq Jul 2008]

Gene Symbol:   TTYH3
Gene Name:   tweety homolog 3 (Drosophila)
Aliases:   KIAA1691
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000136295
Entrez:   80727
Chromosome Mapping:   7p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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