PrimePCR™ Probe Assay: ISCU, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ISCU, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ISCU, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051918
Assay Design:   exonic
Chromosome Location:   12:108959098-108959202question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000535729 ENST00000431221 ENST00000547005 ENST00000311893 ENST00000392807 ENST00000539593 ENST00000338291

Gene Information

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron and are created via several steps that include cysteine desulfurases iron donors chaperones and scaffold proteins. This gene encodes the two isomeric forms ISCU1 and ISCU2 of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. [provided by RefSeq Jul 2008]

Gene Symbol:   ISCU
Gene Name:   iron-sulfur cluster scaffold homolog (E. coli)
Aliases:   2310020H20Rik, HML, ISU2, MGC74517, NIFU, NIFUN, hnifU
RefSeq:   NC_000012.11 NG_011857.1 NG_012155.1 NT_029419.12
Ensembl:   ENSG00000136003
Entrez:   23479
Chromosome Mapping:   12q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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