PrimePCR™ Probe Assay: ANKRD36, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ANKRD36, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ANKRD36, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051915
Assay Design:   exonic
Chromosome Location:   2:97779292-97779493question
Amplicon Length:   172
Splice Variants Targeted:   ENST00000420699 ENST00000461153 ENST00000373913 ENST00000373907 ENST00000401952 ENST00000311117 ENST00000476671 ENST00000334877 ENST00000257745 ENST00000334914 ENST00000338432 ENST00000537279 ENST00000377848 ENST00000377854 ENST00000543201 ENST00000355327 ENST00000261862 ENST00000594949 ENST00000595745 ENST00000597268

Gene Information

Description Not Available

Gene Symbol:   ANKRD36
Gene Name:   ankyrin repeat domain 36
Aliases:   FLJ33607, KIAA1641, MGC167812, UNQ2430
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000135976
Entrez:   375248
Chromosome Mapping:   2q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.040000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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