PrimePCR™ Probe Assay: RC3H1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RC3H1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RC3H1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051900
Assay Design:   exonic
Chromosome Location:   1:173931091-173931232question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000367696 ENST00000367694 ENST00000258349

Gene Information

RC3H1 or roquin encodes a highly conserved member of the RING type ubiquitin ligase protein family (Vinuesa et al. 2005 [PubMed 15917799]). The roquin protein is distinguished by the presence of a CCCH zinc finger found in RNA-binding proteins and localization to cytosolic RNA granules implicated in regulating mRNA translation and stability.[supplied by OMIM Mar 2008]

Gene Symbol:   RC3H1
Gene Name:   ring finger and CCCH-type domains 1
Aliases:   KIAA2025, RNF198, ROQUIN
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000135870
Entrez:   149041
Chromosome Mapping:   1q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.550000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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