PrimePCR™ Probe Assay: GRP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GRP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GRP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051814
Assay Design:   exonic
Chromosome Location:   18:56897670-56897795question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000256857 ENST00000529320 ENST00000420468 ENST00000456142 ENST00000530323

Gene Information

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. Its preproprotein following cleavage of a signal peptide is further processed to produce either the 27 aa gastrin-releasing peptide or the 10 aa neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems including release of gastrointestinal hormones smooth muscle cell contraction and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung colon stomach pancreas breast and prostate. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   GRP
Gene Name:   gastrin-releasing peptide
Aliases:   BN, GRP-10, preproGRP, proGRP
RefSeq:   NC_000018.9 NT_025028.14
Ensembl:   ENSG00000134443
Entrez:   2922
Chromosome Mapping:   18q21.1-q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.870000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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