PrimePCR™ Probe Assay: CTIF, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051787
Assay Design:   exonic
Chromosome Location:   18:46146004-46163012question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000379441 ENST00000551812 ENST00000549884 ENST00000179765 ENST00000582330 ENST00000335464 ENST00000321599 ENST00000468463 ENST00000409457 ENST00000304032 ENST00000336665 ENST00000371746 ENST00000371748 ENST00000256413 ENST00000382998 ENST00000591412 ENST00000591387 ENST00000587752 ENST00000588345 ENST00000397081 ENST00000409036 ENST00000264111 ENST00000268296 ENST00000562522 ENST00000262418 ENST00000252252 ENST00000353139 ENST00000360679 ENST00000394406 ENST00000335166 ENST00000394480 ENST

Gene Information

CTIF is a component of the CBP80 (NCBP1; MIM 600469)/CBP20 (NCBP2; MIM 605133) translation initiation complex that binds cotranscriptionally to the cap end of nascent mRNA. The CBP80/CBP20 complex is involved in a simultaneous editing and translation step that recognizes premature termination codons (PTCs) in mRNAs and directs PTC-containing mRNAs toward nonsense-mediated decay (NMD). On mRNAs without PTCs the CBP80/CBP20 complex is replaced with cytoplasmic mRNA cap-binding proteins including EIF4G (MIM 600495) and steady-state translation of the mRNAs resumes in the cytoplasm (Kim et al. 2009 [PubMed 19648179]).[supplied by OMIM Dec 2009]

Gene Symbol:   CTIF
Gene Name:   CBP80/20-dependent translation initiation factor
Aliases:   Gm672, KIAA0427
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000134030
Entrez:   9811
UniGene:   Hs.145230
Chromosome Mapping:   18q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.470000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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