PrimePCR™ Probe Assay: NTS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NTS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NTS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051764
Assay Design:   exonic
Chromosome Location:   12:86276080-86276223question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000551529 ENST00000256010 ENST00000550879

Gene Information

This gene encodes a common precursor for two peptides neuromedin N and neurotensin. Neurotensin is a secreted tridecapeptide which is widely distributed throughout the central nervous system and may function as a neurotransmitter or a neuromodulator. It may be involved in dopamine-associated pathophysiological events in the maintenance of gut structure and function and in the regulation of fat metabolism. Tissue-specific processing may lead to the formation in some tissues of larger forms of neuromedin N and neurotensin. The large forms may represent more stable peptides that are also biologically active. [provided by RefSeq Jul 2008]

Gene Symbol:   NTS
Gene Name:   neurotensin
Aliases:   NMN-125, NN, NT, NT/N, NTS1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000133636
Entrez:   4922
Chromosome Mapping:   12q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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