PrimePCR™ Probe Assay: GUCY2D, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051706
Assay Design:   exonic
Chromosome Location:   17:7918212-7918341question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000254854

Gene Information

This gene encodes a retina-specific guanylate cyclase which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain a single membrane spanning domain a kinase homology domain and a guanylyl cyclase catalytic domain. In contrast to other membrane guanylyl cyclases this enzyme is not activated by natriuretic peptides. Mutations in this gene result in Leber congenital amaurosis and cone-rod dystrophy-6 diseases. [provided by RefSeq Dec 2008]

Gene Symbol:   GUCY2D
Gene Name:   guanylate cyclase 2D, membrane (retina-specific)
Aliases:   CORD5, CORD6, CYGD, GUC1A4, GUC2D, LCA, LCA1, RCD2, RETGC-1, ROS-GC1, ROSGC, retGC
RefSeq:   NC_000017.10 NG_009092.1 NT_010718.16
Ensembl:   ENSG00000132518
Entrez:   3000
UniGene:   Hs.592109
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.880000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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