PrimePCR™ Probe Assay: CC2D1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CC2D1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CC2D1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051681
Assay Design:   exonic
Chromosome Location:   19:14017291-14020710question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000421367 ENST00000318003 ENST00000589606 ENST00000411589 ENST00000442938 ENST00000354064 ENST00000533102 ENST00000313236 ENST00000524725 ENST00000359862 ENST00000367988 ENST00000367987 ENST00000258796 ENST00000403167 ENST00000398646 ENST00000314412 ENST00000372980 ENST00000369872 ENST00000306467 ENST00000508442 ENST00000328236 ENST00000306481 ENST00000395431 ENST00000259455 ENST00000216181 ENST00000442510 ENST00000367367 ENST00000348564 ENST00000367376 ENST00000352140 ENST00000594404 ENST

Gene Information

This gene encodes a transcriptional repressor that binds to a conserved 14-bp 5'-repressor element and regulates expression of the 5-hydroxytryptamine (serotonin) receptor 1A gene in neuronal cells. The DNA binding and transcriptional repressor activities of the protein are inhibited by calcium. A mutation in this gene results in nonsyndromic mental retardation-3.[provided by RefSeq Oct 2009]

Gene Symbol:   CC2D1A
Gene Name:   coiled-coil and C2 domain containing 1A
Aliases:   FLJ20241, FLJ41160, FREUD-1, Freud-1/Aki1, MRT3
RefSeq:   NC_000019.9 NG_013089.1 NT_011295.11
Ensembl:   ENSG00000132024
Entrez:   54862
Chromosome Mapping:   19p13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.030000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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