PrimePCR™ Probe Assay: DNAJB1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051679
Assay Design:   exonic
Chromosome Location:   19:14627674-14627766question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000254322 ENST00000396969 ENST00000598235 ENST00000595139 ENST00000596853 ENST00000595992 ENST00000594099 ENST00000601533 ENST00000598692 ENST00000596075

Gene Information

Description Not Available

Gene Symbol:   DNAJB1
Gene Name:   DnaJ (Hsp40) homolog, subfamily B, member 1
Aliases:   HSPF1, Hdj1, Hsp40, RSPH16B, Sis1
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000132002
Entrez:   3337
UniGene:   Hs.515210
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.810000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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