PrimePCR™ Probe Assay: RBM8A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RBM8A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RBM8A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051672
Assay Design:   exonic
Chromosome Location:   1:145508483-145508936question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000330165 ENST00000369307 ENST00000583313 ENST00000578414

Gene Information

This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present thus coupling pre- and post-mRNA splicing events. Previously it was thought that two genes encode this protein RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein and this gene also uses multiple polyadenylation sites. [provided by RefSeq Jul 2008]

Gene Symbol:   RBM8A
Gene Name:   RNA binding motif protein 8A
Aliases:   BOV-1A, BOV-1B, BOV-1C, MDS014, RBM8, RBM8B, Y14, ZNRP, ZRNP1
RefSeq:   NC_000001.10 NT_167185.1
Ensembl:   ENSG00000131795
Entrez:   9939
Chromosome Mapping:   1q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.130000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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