PrimePCR™ Probe Assay: PIAS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PIAS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PIAS3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051671
Assay Design:   exonic
Chromosome Location:   1:145578125-145578227question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000375295 ENST00000546746 ENST00000595535 ENST00000352632 ENST00000598249 ENST00000392023 ENST00000338932 ENST00000392025 ENST00000449788 ENST00000546399 ENST00000225831 ENST00000411583 ENST00000548530 ENST00000250383 ENST00000344777 ENST00000383325 ENST00000549812 ENST00000602553 ENST00000377626 ENST00000441795 ENST00000268281 ENST00000418068 ENST00000569305 ENST00000428956 ENST00000498271 ENST00000393046 ENST00000393045 ENST00000369298 ENST00000369299 ENST00000435363 ENST00000425700 ENST

Gene Information

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   PIAS3
Gene Name:   protein inhibitor of activated STAT, 3
Aliases:   FLJ14651, ZMIZ5
RefSeq:   NC_000001.10 NT_167185.1
Ensembl:   ENSG00000131788
Entrez:   10401
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.960000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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