PrimePCR™ Probe Assay: HAUS8, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051637
Assay Design:   exonic
Chromosome Location:   19:17163653-17163747question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000253669 ENST00000448593 ENST00000593360

Gene Information

HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex or HAUS complex. The augmin complex was first identified in Drosophila and its name comes from the Latin verb 'augmentare' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al. 2008 [PubMed 18443220]; Uehara et al. 2009 [PubMed 19369198]).[supplied by OMIM Jun 2010]

Gene Symbol:   HAUS8
Gene Name:   HAUS augmin-like complex, subunit 8
Aliases:   DGT4, HICE1, MGC20533, NY-SAR-48
RefSeq:   NC_000019.9 NG_013068.1 NT_011295.11
Ensembl:   ENSG00000131351
Entrez:   93323
UniGene:   Hs.404088
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.430000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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