PrimePCR™ Probe Assay: SYNE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SYNE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SYNE1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051617
Assay Design:   exonic
Chromosome Location:   6:152792733-152792838question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000367255 ENST00000423061 ENST00000367253 ENST00000367248 ENST00000413186 ENST00000495090 ENST00000466159 ENST00000537750 ENST00000265368 ENST00000448038 ENST00000341594

Gene Information

This gene encodes a spectrin repeat containing protein expressed in skeletal and smooth muscle and peripheral blood lymphocytes that localizes to the nuclear membrane. Mutations in this gene have been associated with autosomal recessive spinocerebellar ataxia 8 also referred to as autosomal recessive cerebellar ataxia type 1 or recessive ataxia of Beauce. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   SYNE1
Gene Name:   spectrin repeat containing, nuclear envelope 1
Aliases:   8B, ARCA1, C6orf98, CPG2, DKFZp781J13156, EDMD4, FLJ30878, FLJ41140, KIAA0796, KIAA1262, KIAA1756, MYNE1, SCAR8, dJ45H2.2
RefSeq:   NC_000006.11 NT_025741.15 NG_012855.1
Ensembl:   ENSG00000131018
Entrez:   23345
Chromosome Mapping:   6q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.580000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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