PrimePCR™ Probe Assay: ANAPC13, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051508
Assay Design:   exonic
Chromosome Location:   3:134196918-134197031question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000510994 ENST00000354910 ENST00000514612 ENST00000511751

Gene Information

This gene encodes a component of the anaphase promoting complex a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The encoded protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex. Pseudogenes and splice variants have been found for this gene; however the biological validity of some of the splice variants has not been determined. [provided by RefSeq Nov 2008]

Gene Symbol:   ANAPC13
Gene Name:   anaphase promoting complex subunit 13
Aliases:   APC13, DKFZp566D193, SWM1
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000129055
Entrez:   25847
UniGene:   Hs.106909
Chromosome Mapping:   3q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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