PrimePCR™ Probe Assay: IL17B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL17B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL17B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051452
Assay Design:   exonic
Chromosome Location:   5:148753927-148754076question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000573076 ENST00000438447 ENST00000282493 ENST00000261796 ENST00000543010 ENST00000391721 ENST00000339757 ENST00000427260 ENST00000263437 ENST00000448584 ENST00000537859 ENST00000538819 ENST00000024061 ENST00000481105 ENST00000571455 ENST00000576228 ENST00000570593 ENST00000574207 ENST00000571222 ENST00000574136 ENST00000576821 ENST00000571435 ENST00000571477 ENST00000573475 ENST00000574477 ENST00000572900 ENST00000572578 ENST00000570685 ENST00000575196 ENST00000574837 ENST00000576515 ENST

Gene Information

The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. [provided by RefSeq Jul 2008]

Gene Symbol:   IL17B
Gene Name:   interleukin 17B
Aliases:   IL-17B, IL-20, MGC138900, MGC138901, ZCYTO7
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000127743
Entrez:   27190
Chromosome Mapping:   5q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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