PrimePCR™ Probe Assay: PTPRB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTPRB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTPRB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051441
Assay Design:   exonic
Chromosome Location:   12:71002853-71002977question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000334414 ENST00000550358 ENST00000538708 ENST00000550857 ENST00000261266 ENST00000551525 ENST00000548122 ENST00000547715 ENST00000451516

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP contains an extracellular domain a single transmembrane segment and one intracytoplasmic catalytic domain thus belongs to receptor type PTP. The extracellular region of this PTP is composed of multiple fibronectin type_III repeats which was shown to interact with neuronal receptor and cell adhesion molecules such as contactin and tenascin C. This protein was also found to interact with sodium channels and thus may regulate sodium channels by altering tyrosine phosphorylation status. The functions of the interaction partners of this protein implicate the roles of this PTP in cell adhesion neurite growth and neuronal differentiation. Alternate transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq May 2011]

Gene Symbol:   PTPRB
Gene Name:   protein tyrosine phosphatase, receptor type, B
Aliases:   DKFZp686E2262, DKFZp686H15164, FLJ44133, HPTP-BETA, HPTPB, MGC142023, MGC59935, PTPB, R-PTP-BETA, VEPTP
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000127329
Entrez:   5787
Chromosome Mapping:   12q15-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.350000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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