PrimePCR™ Probe Assay: PAX8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PAX8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PAX8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051351
Assay Design:   exonic
Chromosome Location:   2:113976174-113984740question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000477737 ENST00000292478 ENST00000263335 ENST00000397647 ENST00000348715 ENST00000429538 ENST00000465084 ENST00000497038 ENST00000468980 ENST00000263334 ENST00000456124 ENST00000435221 ENST00000361050 ENST00000327031 ENST00000545457 ENST00000579294 ENST00000379450 ENST00000434326 ENST00000402157 ENST00000299550 ENST00000508802 ENST00000261439 ENST00000571335 ENST00000436810 ENST00000270570 ENST00000395585 ENST00000457293 ENST00000542886 ENST00000282470 ENST00000503414 ENST00000418378 ENST

Gene Information

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain an octapeptide and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Mar 2010]

Gene Symbol:   PAX8
Gene Name:   paired box 8
Aliases:   Not Available
RefSeq:   NC_000002.11 NT_022135.16 NG_012384.1
Ensembl:   ENSG00000125618
Entrez:   7849
Chromosome Mapping:   2q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.460000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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