PrimePCR™ Probe Assay: AHNAK, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051334
Assay Design:   exonic
Chromosome Location:   11:62302576-62303423question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000449771 ENST00000549151 ENST00000389212 ENST00000548919 ENST00000395358 ENST00000466322 ENST00000405493 ENST00000171000 ENST00000446957 ENST00000269554 ENST00000348261 ENST00000565831 ENST00000358590 ENST00000265074 ENST00000339042 ENST00000326958 ENST00000334011 ENST00000356865 ENST00000380548 ENST00000380545 ENST00000380538 ENST00000530124 ENST00000257247 ENST00000533365 ENST00000378024 ENST00000530285 ENST00000528508 ENST00000531324 ENST00000372315 ENST00000243964 ENST00000454036 ENST

Gene Information

Description Not Available

Gene Symbol:   AHNAK
Gene Name:   AHNAK nucleoprotein
Aliases:   AHNAKRS, MGC5395
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000124942
Entrez:   79026
UniGene:   Hs.502756
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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