This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SERPINA7, Human
PrimePCR™ Template for Probe Assay: SERPINA7, Human
There are three proteins including thyroxine-binding globulin (TBG) transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 353 -triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein TBG in serum. It belongs to the serpin family in genomics but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency which has been classified as partial deficiency complete deficiency and excess based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found but the full-length nature of these variants has not been determined.[provided by RefSeq May 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.