PrimePCR™ Probe Assay: ATP7B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ATP7B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP7B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051240
Assay Design:   exonic
Chromosome Location:   13:52516573-52516690question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000242839 ENST00000400366 ENST00000344297 ENST00000448424 ENST00000400370 ENST00000418097 ENST00000417240

Gene Information

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains an ATPase consensus sequence a hinge domain a phosphorylation site and at least 2 putative copper-binding sites. This protein functions as a monomer exporting copper out of the cells such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants encoding different isoforms with distinct cellular localizations have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq Jul 2008]

Gene Symbol:   ATP7B
Gene Name:   ATPase, Cu++ transporting, beta polypeptide
Aliases:   PWD, WC1, WD, WND
RefSeq:   NG_028038.1 NC_000013.10 NG_008806.1 NT_024524.14
Ensembl:   ENSG00000123191
Entrez:   540
Chromosome Mapping:   13q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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