PrimePCR™ Probe Assay: DNAI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DNAI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DNAI1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051224
Assay Design:   exonic
Chromosome Location:   9:34485220-34489321question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000437363 ENST00000242317 ENST00000545019

Gene Information

The inner- and outer-arm dyneins which bridge between the doublet microtubules in axonemes are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains thought to form the base of the dynein arm help mediate attachment and may also participate in regulating dynein activity. This gene encodes an intermediate chain dynein belonging to the large family of motor proteins. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   DNAI1
Gene Name:   dynein, axonemal, intermediate chain 1
Aliases:   CILD1, ICS, ICS1, MGC26204, PCD
RefSeq:   NG_027971.1 NC_000009.11 NG_008127.1 NT_008413.18
Ensembl:   ENSG00000122735
Entrez:   27019
Chromosome Mapping:   9p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.510000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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