PrimePCR™ Probe Assay: NPY, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051215
Assay Design:   exonic
Chromosome Location:   7:24329198-24331419question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000242152 ENST00000407573 ENST00000405982

Gene Information

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes including cortical excitability stress response food intake circadian rhythms and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase activate mitogen-activated protein kinase (MAPK) regulate intracellular calcium levels and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels higher alcohol consumption and may be a risk factor for various metabolic and cardiovascular diseases. [provided by RefSeq Nov 2009]

Gene Symbol:   NPY
Gene Name:   neuropeptide Y
Aliases:   PYY4
RefSeq:   NC_000007.13 NG_016148.1 NT_007819.17
Ensembl:   ENSG00000122585
Entrez:   4852
UniGene:   Hs.1832
Chromosome Mapping:   7p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.900000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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