PrimePCR™ Probe Assay: NXPH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NXPH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NXPH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051214
Assay Design:   exonic
Chromosome Location:   7:8790661-8790781question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000405863 ENST00000438764 ENST00000429542 ENST00000602349

Gene Information

This gene is a member of the neurexophilin family and encodes a secreted protein with a variable N-terminal domain a highly conserved N-glycosylated central domain a short linker region and a cysteine-rich C-terminal domain. This protein forms a very tight complex with alpha neurexins a group of proteins that promote adhesion between dendrites and axons. [provided by RefSeq Jul 2008]

Gene Symbol:   NXPH1
Gene Name:   neurexophilin 1
Aliases:   NPH1
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000122584
Entrez:   30010
Chromosome Mapping:   7p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.430000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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