PrimePCR™ Probe Assay: TCP1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051120
Assay Design:   exonic
Chromosome Location:   6:160210462-160210551question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000321394 ENST00000544255 ENST00000420894 ENST00000392168 ENST00000539756 ENST00000538128 ENST00000537390

Gene Information

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT) also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins including actin and tubulin. Alternate transcriptional splice variants of this gene encoding different isoforms have been characterized. In addition three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq Jun 2010]

Gene Symbol:   TCP1
Gene Name:   t-complex 1
Aliases:   CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha
RefSeq:   NC_000006.11 NG_023389.1 NT_025741.15
Ensembl:   ENSG00000120438
Entrez:   6950
UniGene:   Hs.363137
Chromosome Mapping:   6q25.3-q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.110000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download