PrimePCR™ Probe Assay: PPP1R3C, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051093
Assay Design:   exonic
Chromosome Location:   10:93388288-93388413question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000238994

Gene Information

This gene encodes a regulatory subunit of protein phosphatase-1 (PP1). PP1 catalyzes reversible protein phosphorylation which is important in a wide range of cellular activities: neuronal muscular RNA splicing protein synthesis cell death and glycogen metabolism to name just a few. By interacting with different regulatory subunits PP1 is directed to different parts of the cell to different substrates or to respond to extracellular signals. [provided by RefSeq Oct 2011]

Gene Symbol:   PPP1R3C
Gene Name:   protein phosphatase 1, regulatory (inhibitor) subunit 3C
Aliases:   PPP1R5
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000119938
Entrez:   5507
UniGene:   Hs.731672
Chromosome Mapping:   10q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.800000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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