PrimePCR™ Probe Assay: ATL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051088
Assay Design:   exonic
Chromosome Location:   2:38537463-38537588question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000378954 ENST00000406122 ENST00000402054 ENST00000419554 ENST00000452935 ENST00000449130 ENST00000443098 ENST00000539122 ENST00000332337 ENST00000546051

Gene Information

Description Not Available

Gene Symbol:   ATL2
Gene Name:   atlastin GTPase 2
Aliases:   ARL3IP2, ARL6IP2, FLJ23293, atlastin2
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000119787
Entrez:   64225
UniGene:   Hs.727652
Chromosome Mapping:   2p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.280000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download