PrimePCR™ Probe Assay: ZNF430, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF430, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF430, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051050
Assay Design:   exonic
Chromosome Location:   19:21216898-21239447question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000476120 ENST00000545510 ENST00000319636 ENST00000261560 ENST00000599548 ENST00000594110 ENST00000595401 ENST00000456783 ENST00000486528 ENST00000496398 ENST00000525354 ENST00000334589 ENST00000531821 ENST00000594466 ENST00000575986 ENST00000322487 ENST00000339642 ENST00000357002 ENST00000573540

Gene Information

Description Not Available

Gene Symbol:   ZNF430
Gene Name:   zinc finger protein 430
Aliases:   DKFZp762K012, FLJ13659, MGC133232
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000118620
Entrez:   80264
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.400000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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