PrimePCR™ Probe Assay: FHL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FHL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FHL2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050892
Assay Design:   exonic
Chromosome Location:   2:105977784-105977882question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000409177 ENST00000344213 ENST00000393353 ENST00000393352 ENST00000322142 ENST00000409807 ENST00000408995 ENST00000358129 ENST00000336660

Gene Information

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved tandemly arranged zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Aug 2011]

Gene Symbol:   FHL2
Gene Name:   four and a half LIM domains 2
Aliases:   AAG11, DRAL, FHL-2, SLIM-3, SLIM3
RefSeq:   NC_000002.11 NT_022171.15 NG_008844.1
Ensembl:   ENSG00000115641
Entrez:   2274
Chromosome Mapping:   2q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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