PrimePCR™ Probe Assay: PDE1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PDE1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDE1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050849
Assay Design:   exonic
Chromosome Location:   2:183088670-183094841question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000435564 ENST00000409365 ENST00000410103 ENST00000331935 ENST00000346717 ENST00000536095 ENST00000358139 ENST00000351439 ENST00000456212

Gene Information

Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family such as PDE1A are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al. 2001 [PubMed 11342109]; Fidock et al. 2002 [PubMed 11747989]).[supplied by OMIM Oct 2009]

Gene Symbol:   PDE1A
Gene Name:   phosphodiesterase 1A, calmodulin-dependent
Aliases:   HCAM1, HSPDE1A, MGC26303
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000115252
Entrez:   5136
Chromosome Mapping:   2q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.150000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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