PrimePCR™ Probe Assay: GNB4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050814
Assay Design:   exonic
Chromosome Location:   3:179119009-179122984question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000232564 ENST00000466899 ENST00000468623

Gene Information

Heterotrimeric guanine nucleotide-binding proteins (G proteins) which integrate signals between receptors and effector proteins are composed of an alpha a beta and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits as well as of certain signal transduction receptors and effectors. [provided by RefSeq Jul 2008]

Gene Symbol:   GNB4
Gene Name:   guanine nucleotide binding protein (G protein), beta polypeptide 4
Aliases:   Not Available
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114450
Entrez:   59345
UniGene:   Hs.173030
Chromosome Mapping:   3q26.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.600000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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