PrimePCR™ Probe Assay: HYAL1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050807
Assay Design:   exonic
Chromosome Location:   3:50339496-50339636question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000571551 ENST00000570967 ENST00000576782 ENST00000572970 ENST00000575445 ENST00000570958 ENST00000395144 ENST00000266031 ENST00000320295 ENST00000395143 ENST00000457214 ENST00000447605

Gene Information

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   HYAL1
Gene Name:   hyaluronoglucosaminidase 1
Aliases:   HYAL-1, LUCA1, MGC45987, NAT6
RefSeq:   NC_000003.11 NT_022517.18 NG_009295.1
Ensembl:   ENSG00000114378
Entrez:   3373
UniGene:   Hs.75619
Chromosome Mapping:   3p21.3-p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.530000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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