PrimePCR™ Probe Assay: TBCCD1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050785
Assay Design:   exonic
Chromosome Location:   3:186274418-186274525question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000331243 ENST00000295092 ENST00000354532 ENST00000599846 ENST00000347545 ENST00000337665 ENST00000258770 ENST00000361278 ENST00000395655 ENST00000494076 ENST00000432126 ENST00000338733 ENST00000446782 ENST00000413695 ENST00000424280 ENST00000300632 ENST00000337883 ENST00000418738 ENST00000381389 ENST00000381392 ENST00000434045 ENST00000416167 ENST00000381406 ENST00000381395

Gene Information

Description Not Available

Gene Symbol:   TBCCD1
Gene Name:   TBCC domain containing 1
Aliases:   FLJ10560
RefSeq:   NC_000003.11 NG_009829.1 NT_005612.16
Ensembl:   ENSG00000113838
Entrez:   55171
UniGene:   Hs.518469
Chromosome Mapping:   3q27.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.230000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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