PrimePCR™ Probe Assay: COX7A2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COX7A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COX7A2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050714
Assay Design:   exonic
Chromosome Location:   6:75947542-75947681question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000370081 ENST00000370089 ENST00000509698 ENST00000460985 ENST00000472311 ENST00000230459

Gene Information

Cytochrome c oxidase the terminal component of the mitochondrial respiratory chain catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2 subunit VIIa includes polypeptide 1 (muscle isoform) which is present only in muscle tissues and a related protein which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14. [provided by RefSeq Oct 2009]

Gene Symbol:   COX7A2
Gene Name:   cytochrome c oxidase subunit VIIa polypeptide 2 (liver)
Aliases:   COX7AL, COX7AL1, COXVIIAL, COXVIIa-L, MGC118950, MGC118951, MGC118952, MGC126875, MGC126877, VIIAL
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000112695
Entrez:   1347
Chromosome Mapping:   6q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.160000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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