PrimePCR™ Probe Assay: COL9A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL9A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL9A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050693
Assay Design:   exonic
Chromosome Location:   6:70925969-70926100question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000357250 ENST00000320755 ENST00000370499

Gene Information

This gene encodes one of the three alpha chains of type IX collagen which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules a heterotrimeric molecule and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans with multiple epiphyseal dysplasia 6 a form of chondrodysplasia and with Stickler syndrome a disease characterized by ophthalmic orofacial articular and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   COL9A1
Gene Name:   collagen, type IX, alpha 1
Aliases:   DJ149L1.1.2, EDM6, FLJ40263, MED
RefSeq:   NC_000006.11 NG_011654.1 NT_007299.13
Ensembl:   ENSG00000112280
Entrez:   1297
Chromosome Mapping:   6q12-q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.640000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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