PrimePCR™ Probe Assay: USP5, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050651
Assay Design:   exonic
Chromosome Location:   12:6961366-6964585question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000264659 ENST00000578925 ENST00000445265 ENST00000323668 ENST00000451292 ENST00000377797 ENST00000229268 ENST00000389231 ENST00000542087 ENST00000581317 ENST00000570888 ENST00000595281 ENST00000597018 ENST00000596774

Gene Information

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure receptor function and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism starting at the proximal end of the chain (Wilkinson et al. 1995 [PubMed 7578059]).[supplied by OMIM Mar 2010]

Gene Symbol:   USP5
Gene Name:   ubiquitin specific peptidase 5 (isopeptidase T)
Aliases:   ISOT
RefSeq:   NC_000012.11 NG_011948.1 NT_009759.16
Ensembl:   ENSG00000111667
Entrez:   8078
UniGene:   Hs.631661
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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