PrimePCR™ Probe Assay: COPS7A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: COPS7A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COPS7A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050650
Assay Design:   exonic
Chromosome Location:   12:6837392-6838424question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000543155 ENST00000229251 ENST00000539735 ENST00000538410 ENST00000544725 ENST00000534947 ENST00000541866 ENST00000534877 ENST00000538753

Gene Information

Description Not Available

Gene Symbol:   COPS7A
Gene Name:   COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis)
Aliases:   CSN7A, MGC110877
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000111652
Entrez:   50813
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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