PrimePCR™ Probe Assay: LEPREL2, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050593
Assay Design:   exonic
Chromosome Location:   12:6948912-6949005question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000470125 ENST00000577830 ENST00000290510 ENST00000396725 ENST00000597750 ENST00000595716

Gene Information

LEPREL2 belongs to a family of collagen prolyl hydroxylases required for proper collagen biosynthesis folding and assembly (Vranka et al. 2004 [PubMed 15044469]).[supplied by OMIM Mar 2008]

Gene Symbol:   LEPREL2
Gene Name:   leprecan-like 2
Aliases:   GRCB, HSU47926, P3H3
RefSeq:   NC_000012.11 NG_009100.1 NT_009759.16
Ensembl:   ENSG00000110811
Entrez:   10536
UniGene:   Hs.631655
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.500000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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