PrimePCR™ Probe Assay: MDK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MDK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MDK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050579
Assay Design:   exonic
Chromosome Location:   11:46404288-46405149question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000405308 ENST00000359803 ENST00000395569 ENST00000395566 ENST00000407067 ENST00000395565

Gene Information

Midkine is a retinoic acid-responsive heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis cell growth and cell migration. Midkine is also expressed in several carcinomas suggesting that it may play a role in tumorigenesis perhaps through its effects on angiogenesis (summary by Reynolds et al. 2004 [PubMed 15197188]).[supplied by OMIM Nov 2010]

Gene Symbol:   MDK
Gene Name:   midkine (neurite growth-promoting factor 2)
Aliases:   FLJ27379, MK, NEGF2
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000110492
Entrez:   4192
Chromosome Mapping:   11p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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