PrimePCR™ Probe Assay: WHSC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WHSC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WHSC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050549
Assay Design:   exonic
Chromosome Location:   4:1901118-1902447question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000508803 ENST00000507820 ENST00000514045 ENST00000515806 ENST00000420906 ENST00000382895 ENST00000503128 ENST00000509115 ENST00000382891 ENST00000382892 ENST00000398261 ENST00000436793

Gene Information

This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain an HMG box a SET domain and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates hence not represented as reference sequences. [provided by RefSeq Jul 2008]

Gene Symbol:   WHSC1
Gene Name:   Wolf-Hirschhorn syndrome candidate 1
Aliases:   FLJ23286, KIAA1090, MGC176638, MMSET, NSD2, REIIBP, TRX5, WHS
RefSeq:   NC_000004.11 NG_009232.1 NG_009269.1 NT_006051.18
Ensembl:   ENSG00000109685
Entrez:   7468
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.610000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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