PrimePCR™ Probe Assay: FOXN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FOXN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FOXN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050524
Assay Design:   exonic
Chromosome Location:   17:26851102-26851731question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000582057 ENST00000577642 ENST00000584474 ENST00000490882 ENST00000295956 ENST00000358537 ENST00000429972 ENST00000493452 ENST00000348383 ENST00000357272 ENST00000419752 ENST00000577936 ENST00000579795 ENST00000226247 ENST00000256861 ENST00000298441

Gene Information

Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency the skin disorder congenital alopecia and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq Jul 2008]

Gene Symbol:   FOXN1
Gene Name:   forkhead box N1
Aliases:   FKHL20, RONU, WHN
RefSeq:   NC_000017.10 NG_007260.1 NT_010799.15
Ensembl:   ENSG00000109101
Entrez:   8456
Chromosome Mapping:   17q11-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.160000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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