PrimePCR™ Probe Assay: CNTNAP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CNTNAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CNTNAP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050507
Assay Design:   exonic
Chromosome Location:   17:40836080-40836210question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000264638

Gene Information

The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1384-amino acid protein also designated p190 or CASPR for 'contactin-associated protein' includes an extracellular domain with several putative protein-protein interaction domains a putative transmembrane domain and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins and this protein may be the signaling subunit of contactin enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq Jan 2009]

Gene Symbol:   CNTNAP1
Gene Name:   contactin associated protein 1
Aliases:   CASPR, CNTNAP, NRXN4, P190
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108797
Entrez:   8506
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.400000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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