PrimePCR™ Probe Assay: POU6F2, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050416
Assay Design:   exonic
Chromosome Location:   7:39046457-39125554question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000403058 ENST00000518318 ENST00000451021 ENST00000524147 ENST00000520104 ENST00000559001

Gene Information

This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain consisting of a POU-specific domain and a homeodomain separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers in a sequence-specific manner. The POU family members are transcriptional regulators many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq Oct 2009]

Gene Symbol:   POU6F2
Gene Name:   POU class 6 homeobox 2
Aliases:   RPF-1, WT5, WTSL
RefSeq:   NC_000007.13 NT_007819.17 NG_016022.1
Ensembl:   ENSG00000106536
Entrez:   11281
UniGene:   Hs.137106
Chromosome Mapping:   7p14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.950000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download