PrimePCR™ Probe Assay: LMBR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LMBR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LMBR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050380
Assay Design:   exonic
Chromosome Location:   7:156476754-156476849question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000353442 ENST00000415428 ENST00000540390 ENST00000354505 ENST00000359422

Gene Information

This gene encodes a member of the LMBR1-like membrane protein family. Another member of this protein family has been shown to be a lipocalin transmembrane receptor. A highly conserved cis-acting regulatory module for the sonic hedgehog gene is located within an intron of this gene. Consequently disruption of this genic region can alter sonic hedgehog expression and affect limb patterning but it is not known if this gene functions directly in limb development. Mutations and chromosomal deletions and rearrangements in this genic region are associated with acheiropody and preaxial polydactyly which likely result from altered sonic hedgehog expression. [provided by RefSeq Jul 2008]

Gene Symbol:   LMBR1
Gene Name:   limb region 1 homolog (mouse)
Aliases:   ACHP, C7orf2, DIF14, FLJ11665, PPD2, TPT
RefSeq:   NC_000007.13 NG_009240.1 NT_007741.14
Ensembl:   ENSG00000105983
Entrez:   64327
Chromosome Mapping:   7q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.370000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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