PrimePCR™ Probe Assay: SUGP1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050352
Assay Design:   exonic
Chromosome Location:   19:19420906-19420996question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000389840 ENST00000585328 ENST00000324695 ENST00000456930 ENST00000433712 ENST00000550422 ENST00000307885 ENST00000357869 ENST00000244763 ENST00000397511 ENST00000489567 ENST00000534851 ENST00000371929 ENST00000355699 ENST00000356589 ENST00000371916 ENST00000268876 ENST00000394570 ENST00000433649 ENST00000378449 ENST00000465301 ENST00000540313 ENST00000308508 ENST00000247001 ENST00000535070 ENST00000334782 ENST00000276297 ENST00000316609 ENST00000511869 ENST00000333493 ENST00000450388 ENST

Gene Information

SF4 is a member of the SURP family of splicing factors.[supplied by OMIM Sep 2003]

Gene Symbol:   SUGP1
Gene Name:   SURP and G patch domain containing 1
Aliases:   DKFZp434E2216, F23858, RBP, SF4
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000105705
Entrez:   57794
UniGene:   Hs.515274
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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