PrimePCR™ Probe Assay: ILVBL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ILVBL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ILVBL, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050313
Assay Design:   exonic
Chromosome Location:   19:15234229-15234331question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000263383 ENST00000534378 ENST00000600984 ENST00000527093 ENST00000533747

Gene Information

The protein encoded by this gene shares similarity with several thiamine pyrophosphate-binding proteins identified in bacteria yeast and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS) which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. [provided by RefSeq Jul 2008]

Gene Symbol:   ILVBL
Gene Name:   ilvB (bacterial acetolactate synthase)-like
Aliases:   209L8, AHAS, ILV2H, MGC1269, MGC19535
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000105135
Entrez:   10994
Chromosome Mapping:   19p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.320000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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