PrimePCR™ Probe Assay: TTC23, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050246
Assay Design:   exonic
Chromosome Location:   15:99768799-99768919question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000372050 ENST00000372047 ENST00000393160 ENST00000542690 ENST00000372062 ENST00000393157 ENST00000160373 ENST00000394132 ENST00000558663 ENST00000394135 ENST00000394129 ENST00000561365 ENST00000560279 ENST00000560860 ENST00000560772 ENST00000558078 ENST00000560235 ENST00000394136 ENST00000262074 ENST00000558613 ENST00000394130 ENST00000596370 ENST00000597233 ENST00000599529 ENST00000598575 ENST00000598093 ENST00000594236 ENST00000597175 ENST00000595154 ENST00000598966 ENST00000597402 ENST

Gene Information

Description Not Available

Gene Symbol:   TTC23
Gene Name:   tetratricopeptide repeat domain 23
Aliases:   FLJ12572, FLJ12939, HCC-8
RefSeq:   NC_000015.9 NT_010274.17 NG_008210.1
Ensembl:   ENSG00000103852
Entrez:   64927
UniGene:   Hs.513195
Chromosome Mapping:   15q26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.520000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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