PrimePCR™ Probe Assay: NUBP1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050230
Assay Design:   exonic
Chromosome Location:   16:10850637-10851864question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000279024 ENST00000555572 ENST00000373381 ENST00000574334 ENST00000283027 ENST00000433392 ENST00000571175 ENST00000269142 ENST00000578121 ENST00000262316 ENST00000250160 ENST00000394196 ENST00000337975 ENST00000536947 ENST00000377981 ENST00000224721

Gene Information

NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins (Nakashima et al. 1999 [PubMed 10486206]).[supplied by OMIM Mar 2008]

Gene Symbol:   NUBP1
Gene Name:   nucleotide binding protein 1 (MinD homolog, E. coli)
Aliases:   MGC117406, MGC130052, MGC130053, NBP, NBP1
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103274
Entrez:   4682
UniGene:   Hs.81469
Chromosome Mapping:   16p13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.650000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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