PrimePCR™ Probe Assay: PCSK1N, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PCSK1N, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCSK1N, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050171
Assay Design:   exonic
Chromosome Location:   X:48689549-48689688question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000536363 ENST00000292530 ENST00000561623 ENST00000356183 ENST00000265134 ENST00000374059 ENST00000362057 ENST00000377633 ENST00000496902 ENST00000222761 ENST00000248244 ENST00000218230 ENST00000481110 ENST00000260795 ENST00000352904 ENST00000340107 ENST00000440486 ENST00000412135 ENST00000381340 ENST00000361547 ENST00000374315 ENST00000354177 ENST00000601463

Gene Information

Members of the subtilisin-like proprotein convertase family process latent precursor proteins into their biologically active products. The protein encoded by this gene appears to function as an endogenous inhibitor of proprotein convertase subtilisin/kexin type 1. [provided by RefSeq Jul 2008]

Gene Symbol:   PCSK1N
Gene Name:   proprotein convertase subtilisin/kexin type 1 inhibitor
Aliases:   PROSAAS, SAAS
RefSeq:   NC_000023.10 NT_079573.4
Ensembl:   ENSG00000102109
Entrez:   27344
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.670000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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